Streptozotocin-induced Diabetes in FVB/N Mice

Six-week-old male FVB/N mice were purchased from the Jackson laboratory. Animal studies were performed following relevant guidelines and regulations approved by the University of South Florida under ARRIVE guidelines. Animals were housed at constant RT (20 ± 1 °C) under a controlled 12-h light to 12-h dark cycle and had free access to water and a standard diet. The mice received a single i.p. injection of streptozotocin (100 mg/kg body weight, in 0.1 mol/l citric acid buffer, pH 4.5; Sigma–Aldrich, St Louis, MO, U.S.A.) to induce diabetes, or citric acid buffer vehicle (non-diabetic mice) (19 (link)–21 (link)). Diabetes was confirmed by measuring blood glucose from the saphenous vein using a glucometer (Roche, Basel, Switzerland). Mice with streptozotocin-induced blood glucose levels >300 mg/dl were included in the diabetic groups.