PET scans were acquired by an Inveon PET scanner (Siemens Medical Solutions, Knoxville, TN, USA). Sprague-Dawley rats were kept under anesthesia with 1–2% (v/v) isoflurane during the scan. The radiotracer (ca. 1 mCi/150–200 µL) was injected via a preinstalled catheter via tail vein. A dynamic scan in 3D list mode was acquired for 60 min. For pretreatment studies, QCA (1 mg/kg) pre-dissolved in 300 µL saline containing 10% ethanol and 5% Tween® 80 was injected at 30 min via the tail vein catheter before the injection of [11C]QCA.
As we previously reported,78 (link),79 (link) the PET dynamic images were reconstructed using ASIPro VW software (Analysis Tools and System Setup/Diagnostics Tool, Siemens Medical Solutions). Volumes of interest, including the whole brain, cerebral cortex, cerebellum, striatum, thalamus and pons were placed using ASIPro software. The radioactivity was decay-corrected and expressed as the standardized uptake value. SUV = (radioactivity per mL tissue/injected radioactivity) × body weight.
As we previously reported,78 (link),79 (link) the PET dynamic images were reconstructed using ASIPro VW software (Analysis Tools and System Setup/Diagnostics Tool, Siemens Medical Solutions). Volumes of interest, including the whole brain, cerebral cortex, cerebellum, striatum, thalamus and pons were placed using ASIPro software. The radioactivity was decay-corrected and expressed as the standardized uptake value. SUV = (radioactivity per mL tissue/injected radioactivity) × body weight.
