To determine the effect of RA on in vitro follicle development, ovarian cortical pieces from prepubertal (n = 9) versus adult (n = 13) cats were incubated separately for 7 d on 1.5% (w/v) agarose gel blocks in protein-free medium within a 24 well culture plate (Corning Incorporated, Corning, NY), as described previously [12 (link)]. The ovarian cortical pieces combined from both ovaries were randomly divided into fresh, non-cultured control or three culture treatment groups. The culture medium (as described above) was supplemented with 0 (control), 1 or 5 μM RA (2–5 cortical pieces/cat/treatment). Cortical pieces of each donor cat were assessed for viability on the same day of sample collection (Day 0) or after 7 d of culture.
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