Quantifying Equine Growth Factors

The TGF-β₁ and PDGF-BB concentrations from the supernatants and lysates of each blood component were determined in duplicate by a sandwich ELISA using commercially available antibodies against human TGF-β₁ (Human TGF-β1, DY240E, R&D Systems, Inc., Minneapolis, MN USA) and PDGF-BB (Human PDGF-BB, DY220, R&D Systems, Inc.). Both ELISAs were performed according to the manufacturer’s instructions. Readings were performed at 450 nm. Both ELISAs were determined with human antibodies because there is a high homology of these growth factors between equines and humans [18 (link),19 (link)]. Further, several equine PRP studies have validated these ELISA kits [6 (link),14 (link)-16 (link)].

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Giraldo C.E., Álvarez M.E, & Carmona J.U. (2015). Effects of sodium citrate and acid citrate dextrose solutions on cell counts and growth factor release from equine pure-platelet rich plasma and pure-platelet rich gel. BMC Veterinary Research, 11, 60.

Publication 2015

DY240E DY220

Antibodies Blood component Elisa Equine Growth factors Humans Pdgf bb Tgf β1

Corresponding Organization :

Other organizations : University of Caldas

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Variable analysis

independent variables

TGF-β1 concentration
PDGF-BB concentration

dependent variables

TGF-β1 concentration in supernatants
TGF-β1 concentration in lysates
PDGF-BB concentration in supernatants
PDGF-BB concentration in lysates

control variables

Human antibodies used for ELISA kits
ELISA kits validated for equine studies

controls

Positive controls: Not explicitly mentioned
Negative controls: Not explicitly mentioned

Annotations

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