Conditional Girk1 Knockout in Mice

Animal experiments were approved by the University of Minnesota Institutional Animal Care and Use Committee. CaMKIICre [B6.Cg-Tg(Camk2a-cre)T29-1Stl/J] and GADCre (B6N.Cg-Gad2^tm2(cre)Zjh/J) lines were purchased from The Jackson Laboratory, and have been maintained by backcrossing against the C57BL/6J strain. Offspring from these crosses were used to generate the Cre(+) and Cre(−) mice used in this study. The generation of conditional CaMKIICre(+):Girk1^fl/fl mice was described previously (Marron Fernandez de Velasco et al., 2017 (link)). Unless specifically noted, males and females were used in all experiments, and groups were balanced by sex. All mice were maintained on a 12 h light/dark cycle, and were provided ad libitum access to food and water.