Liver Triglyceride Quantification

The liver tissue of each mouse was ground into homogenate and centrifuged to obtain the supernatant. The total protein concentration of the supernatant was determined using a BCA protein concentration determination kit G2026 (Servicebio, Wuhan, China) according to the manufacturer’s instructions. The TG content in liver samples was then measured using the TG Determination Kit (GPO-PAP method) (C061, Huili) against the total protein concentration [18 (link)]. In detail, a 250 μL GPO-PAP working solution was mixed with 2.5 μL PBS, standard glycerol (1.7 mmol/L), or supernatant. The mixture was subjected to OD analysis at 510 nm using the automatic biochemistry analyzer. The TG concentration was resultantly determined using the colorimetric method with standard glycerol normalized by PBS.