Spectrophotometric Assay of SOD Activity

To determine SOD activity, the Fluka 19160 test kit (Germany) was used, which is based on the indirect method of nitrotetrazolium blue chloride (NBT). This assay uses xanthine and xanthine oxidase to generate superoxide radicals, which react with 2-(4-)-3-(4-nitrophenol)-5-phenyl tetrazolium chloride to produce a compound, which absorbs light at 450 nm. The inhibition of chromogen production is proportional to the SOD activity present in the sample. The reading was carried out using a spectrophotometer and the results are reported in U/L.

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Moraes L., Dries S.S., Seibert B.S., Linden R, & Perassolo M.S. (2023). Evaluation of oxidative stress markers in ethanol users. Brazilian Journal of Medical and Biological Research, 56, e12465.

Publication 2023

4 nitrophenol Assay Chloride Chromogen Inhibition Light Nitrotetrazolium blue chloride Superoxide radicals Tetrazolium Xanthine Xanthine oxidase

Corresponding Organization :

Other organizations : Universidade Feevale

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Variable analysis

independent variables

None explicitly mentioned

dependent variables

SOD activity

control variables

Xanthine
Xanthine oxidase
2-(4-)-3-(4-nitrophenol)-5-phenyl tetrazolium chloride
Spectrophotometer

controls

Positive control: None mentioned
Negative control: None mentioned

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