After seven days of quarantine period, animals were induced with diabetes through a single intraperitoneal (i.p.) injection of 60 mg/kg of streptozotocin (Sigma-Aldrich, Germany) after 10 h fasting. streptozotocin was dissolved in 0.1 M citrate buffer solution (pH 4.5) and immediately injected. Normal control rats received the same amount of sodium citrate buffer. Three days after streptozotocin injection, a blood sample was drawn from the tail vein and plasma glucose concentration was checked using the Accu-Chek blood glucose test meter (Roche Diagnostic, Germany). Rats with blood sugar higher than 300 mg/dL (more than 90% of injected animals) were considered diabetics (DM type I). No exogenous insulin treatment was given (19 (link)).
Protocol detail
Streptozotocin-Induced Diabetic Rat Model
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Animals
Blood
Blood sugar
Blood test
Buffer
Citrate
Diabetes
Diagnostic
Glucose
Insulin
Plasma
Quarantine
Rats
Sodium citrate
Streptozotocin
Tail
Vein
Corresponding Organization : Shiraz University of Medical Sciences
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Variable analysis
independent variables
- Streptozotocin (STZ) injection (60 mg/kg, i.p.) after 10 h fasting
- Plasma glucose concentration
- Quarantine period (7 days)
- Sodium citrate buffer injection (for normal control group)
- Positive control: Rats with blood sugar higher than 300 mg/dL (more than 90% of injected animals) were considered diabetics (DM type I).
- Negative control: Normal control rats received the same amount of sodium citrate buffer.
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