Generation of NOD.E2-2fl/fl-CD11c.cre Mice

C57BL/6 (B6) and NOD mice were purchased from Taconic (Ejby, Denmark). B6.E2-2^fl/fl-CD11c.cre [23 (link)] were backcrossed to NOD genetic background for >10 generations to generate NOD.E2-2^fl/fl-CD11c.cre. SNP-based scans were used to ensure that all chromosomes, except chromosome 18 carrying the E2-2 locus were of NOD origin. By in depth SNP analysis of chromosome 18 we have limited the region flanking the E2-2 locus to <19 cM corresponding to <24 Mb (S1 Table). All animals were bred and maintained in a specific pathogen-free environment at the animal facilities at Umeå University or Lund University and all strains were maintained by continuous inbreeding. Mice were sacrificed by cervical dislocation. Only female mice were used for experiments.