Fenofibrate Quantification by HPLC

Agilent Technologies 1260 Series liquid chromatography system with Clarity chromatography software was used. Gradient method: mobile phase A ammonium formate 10 mM, pH 3 in water; mobile phase B ammonium formate 10 mM, pH 3 in acetonitrile/water 9:1, time 0, 70%A:30%B, 3 min 0%A:100%B, 4 min 0%A:100%B, 4.5 min 70%A:30%B, total run 8 min. Column × Bridge C18 column/186003108/50 mm × 2.1 mm id. 5 μm was used. Fenofibrate (retention time 3.6 min) calibration curve (n = 6 concentrations) was run for the excipient-free DoE and each excipient concentration, with the lowest linear regression coefficient of 0.9979 for the low chitosan excipient concentration system. The method has been reported previously [13 (link),21 (link)].