Primary antibodies to IRF1 (1:500 dilution, #8478), Src (1:500 dilution, #2123), and phospho-Src (Y416) (1:500 dilution, #6943) were from Cell Signaling Technology; DYKDDDDK (Clone 1E6, 1:1000 dilution, 018–22381) and GAPDH were from Wako (Clone 5A12; 1:5000 dilution, 016-25523); CSNK2B (1:2000 dilution, A301-984A) and CSNK2A2 (1:2000 dilution, A300-199A) were from Bethyl Laboratories; and CSNK2A1 (1:2000 dilution, 10992-1-AP) and AFAP1 (1:500 dilution, 14544-1-AP) were from Proteintech. IRDye 680 or 800 secondary antibodies including #926-32211, #926-32212, #926-32214, #926-68020 and #926-68073 (1:20 000 dilution) were from LI-COR.
CX-4945 (Silmitasertib) was purchased from Selleck. Puromycin and Hygromycin B Gold were from InvivoGen. Pyridone 6 (JAK Inhibitor I) was from Cayman Chemical. All-trans-retinoic acid was from Wako. Recombinant human IFN-γ and CSNK2B were from Pepro Tech and NKMAX, respectively. PSI-7977 (Sofosbuvir) was from ChemScene. Lambda protein phosphatase was obtained from Bio Academia. miR-122 mimics were synthesized by Dharmacon and transfected by electroporation as miRNA/miRNA* duplexes as described (9 (link)). Cell viability was determined using Cell Counting Kit-8 (DOJINDO, Japan) on 96-well plates according to the manufacturer's protocol.
CX-4945 (Silmitasertib) was purchased from Selleck. Puromycin and Hygromycin B Gold were from InvivoGen. Pyridone 6 (JAK Inhibitor I) was from Cayman Chemical. All-trans-retinoic acid was from Wako. Recombinant human IFN-γ and CSNK2B were from Pepro Tech and NKMAX, respectively. PSI-7977 (Sofosbuvir) was from ChemScene. Lambda protein phosphatase was obtained from Bio Academia. miR-122 mimics were synthesized by Dharmacon and transfected by electroporation as miRNA/miRNA* duplexes as described (9 (link)). Cell viability was determined using Cell Counting Kit-8 (DOJINDO, Japan) on 96-well plates according to the manufacturer's protocol.
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