Based on a previously reported method for obtaining thermographic ocular images and measurements [26 (link)], a standard protocol was maintained in all cases in order to reduce the risk of bias. Thermographic imaging was performed under controlled environmental conditions, with a fixed room temperature of 25 °C, and no air drafts. Thermographic images were taken only after at least 20 min of acclimatization to room temperature.
Prior to image capture, the room and core body temperature (per os) were recorded. Thermal images were taken from the same distance of 30 cm. Video and still thermal images were taken using Therm-App Pro TH (Opgal Optronic Industries Ltd. Karmiel, Israel) camera with a 9 mm lens (384 × 288-pixel resolution).
The subjects were instructed to place their head in the slit lamp, and look straight into the camera. During imaging, subjects were requested to breath normally with their mouth and nose covered by a standard surgical face mask (Sion Biotext medical Ltd., Maastricht, The Netherlands). The images were taken consecutively over a 30 s period, capturing the thermographic data at inspirium and expirium. After completion, 20 of the subjects were also asked to repeat the imaging after sealing the superior end of the mask using micropore surgical tape (3M, Saint Paul, MN, USA). For each participant, the right eye was used for analysis.
Prior to image capture, the room and core body temperature (per os) were recorded. Thermal images were taken from the same distance of 30 cm. Video and still thermal images were taken using Therm-App Pro TH (Opgal Optronic Industries Ltd. Karmiel, Israel) camera with a 9 mm lens (384 × 288-pixel resolution).
The subjects were instructed to place their head in the slit lamp, and look straight into the camera. During imaging, subjects were requested to breath normally with their mouth and nose covered by a standard surgical face mask (Sion Biotext medical Ltd., Maastricht, The Netherlands). The images were taken consecutively over a 30 s period, capturing the thermographic data at inspirium and expirium. After completion, 20 of the subjects were also asked to repeat the imaging after sealing the superior end of the mask using micropore surgical tape (3M, Saint Paul, MN, USA). For each participant, the right eye was used for analysis.
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