To investigate the role of Mito-TEMPO in sepsis-induced liver damage, a lipopolysaccharide- (LPS-) induced experimental sepsis mouse model was established by injecting male mice intraperitoneally with LPS (E. coli 0111: B4, Sigma, USA) at a dosage of 5 mg/kg body weight. The control group was injected with the same volume of sterile phosphate-buffered saline (PBS), as in previous studies [26 (link), 27 (link)].
The mice were randomized and divided into three groups: the control group, the LPS group, and the LPS+Mito-TEMPO group (pretreatment with Mito-TEMPO followed by LPS injection). Mito-TEMPO (Sigma, USA) was intraperitoneally injected at a dose of 20 mg/kg body weight 1 h prior to LPS injection. The dosage of Mito-TEMPO is comparable with previous studies [28 (link), 29 (link)]. Animals were euthanized 24 h after LPS administration, and whole blood and liver samples were harvested for analysis.
The mice were randomized and divided into three groups: the control group, the LPS group, and the LPS+Mito-TEMPO group (pretreatment with Mito-TEMPO followed by LPS injection). Mito-TEMPO (Sigma, USA) was intraperitoneally injected at a dose of 20 mg/kg body weight 1 h prior to LPS injection. The dosage of Mito-TEMPO is comparable with previous studies [28 (link), 29 (link)]. Animals were euthanized 24 h after LPS administration, and whole blood and liver samples were harvested for analysis.
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