Quantifying Inflammasome Genes in Mesothelioma

To determine the effect of XMD8-92 treatment on constitutive inflammasome related genes in mesothelioma cells, total RNA was extracted using the Qiagen RNeasy Plus Mini kit as per the manufacturer’s instructions (Qiagen, Germantown, MD). One μg of RNA from each sample was reversed transcribed using Promega AMV Reverse Transcriptase Kit (Promega) as previously reported [4 (link)]. Specific gene expression was quantified using a primer and probe mixture (Applied Biosystems) and 7700 Sequence Prism Detector (Perkin Elmer, Applied Biosystems) [4 (link)].

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Thompson J.K., Shukla A., Leggett A.L., Munson P.B., Miller J.M., MacPherson M.B., Beuschel S.L., Pass H.I, & Shukla A. (2017). Extracellular signal regulated kinase 5 and inflammasome in progression of mesothelioma. Oncotarget, 9(1), 293-305.

Publication 2017

RNeasy Plus Mini kit 7700 Sequence Prism Detector

Cells Gene expression Genes Inflammasome Mesothelioma Primer Prism Promega Reverse transcriptase Xmd8 92

Corresponding Organization : University of Vermont

Other organizations : NYU Langone Health

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Variable analysis

independent variables

XMD8-92 treatment

dependent variables

Constitutive inflammasome related gene expression

control variables

Mesothelioma cells
Total RNA extraction using Qiagen RNeasy Plus Mini kit
RNA reverse transcription using Promega AMV Reverse Transcriptase Kit
Gene expression quantification using primer and probe mixture and 7700 Sequence Prism Detector

controls

No positive or negative controls were explicitly mentioned in the provided information.

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