Homo sapiens Grx2, Grx5, their mutants, E. coli IscS, roGFP2, D. rerio Sirt1, and AtGrxC1 were expressed as His-tagged proteins and purified via immobilized metal affinity chromatrography7 (link),10 (link),66 (link). SDS-PAGE was performed using pre-casted TGX stain-free gels (4–20%, BioRad, Hercules CA, USA) and imaged according to the manufacturers’ instructions. Protein concentration was determined at 280 nm (εGrx5 = 11,585 and εGrx2 = 7,450 M−1 cm−1).
D rerio E coliGelsHomo sapiens Metal Protein Sds page Sirt1 Stain
Corresponding Organization :
Other organizations :
Universitätsmedizin Greifswald, Universität Greifswald, Heinrich Heine University Düsseldorf, University of Bonn, Newcastle University, DKFZ-ZMBH Alliance, German Cancer Research Center, Heidelberg University, Philipps University of Marburg
Protein expression and purification via immobilized metal affinity chromatography
SDS-PAGE analysis
Protein concentration determination at 280 nm
control variables
Pre-casted TGX stain-free gels (4–20%, BioRad, Hercules CA, USA) for SDS-PAGE
Manufacturers' instructions for imaging SDS-PAGE gels
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