Fly Genetics and Molecular Techniques

Drosophila melanogaster stocks and crosses were maintained in humidified incubators at 25°C with 12 hr light-dark cycles. The alleles Nab2^ex3 (null), Nab2^pex41 (precise excision 41; control), and UAS-Flag-Nab2 have been described previously (Pak et al., 2011 (link); Kelly et al., 2014 (link)). Lines from Bloomington Drosophila Stock Center (BDSC): GMR-Gal4 (#1350), elav^C155-Gal4 (#458), msl-2²²⁷ (#5871), msl-2^kmA (#25158), mle⁹ (#5873), roX1^ex6 (#43647), UAS-Mettl3, UAS-Mettl3-RNAi (#80450), fl(2)d² (#36302), vir^2F (#77886). The Mettl3^null, UAS-Mettl3, and Ythdc^ΔN alleles were all kind gifts of J-Y. Roignant. The Nab2^ex3,Mettl3^null and Nab2^ex3, Ythdc^ΔN chromosomes were generated by meiotic recombination and confirmed by genomic PCR. A total of 200 recombinant lines were screened to identify Nab2,Mettl3 double mutants.

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Jalloh B., Lancaster C.L., Rounds J.C., Brown B.E., Leung S.W., Banerjee A., Morton D.J., Bienkowski R.S., Fasken M.B., Kremsky I.J., Tegowski M., Meyer K., Corbett A, & Moberg K. (2023). The Drosophila Nab2 RNA binding protein inhibits m6A methylation and male-specific splicing of Sex lethal transcript in female neuronal tissue. eLife, 12, e64904.

Publication 2023

GMR-Gal4 elavC155-Gal4

Alleles Center lines Chromosomes Drosophila Drosophila melanogaster Genomic Gifts Meiotic Mettl3 Nab2 Recombination Rnai

Corresponding Organization :

Other organizations : Emory University, Emory and Henry College, Duke University

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Variable analysis

independent variables

Nab2^ex3 (null)
Nab2^pex41 (precise excision 41; control)
UAS-Flag-Nab2
Mettl3^null
UAS-Mettl3
Ythdc^ΔN

dependent variables

Not explicitly mentioned

control variables

Drosophila melanogaster stocks and crosses maintained in humidified incubators at 25°C with 12 hr light-dark cycles
Nab2^pex41 (precise excision 41; control)
Bloomington Drosophila Stock Center (BDSC) lines: GMR-Gal4, elav^C155-Gal4, msl-2^227, msl-2^kmA, mle^9, roX1^ex6, UAS-Mettl3, UAS-Mettl3-RNAi, fl(2)d^2, vir^2F

positive controls

Nab2^pex41 (precise excision 41; control)

negative controls

Not explicitly mentioned

Annotations

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