Dipyridamole Solution Preparation and Pretreatment

Dipyridamole solution is prepared as follows: 20 mg Dipyridamole (Sigma-Aldrich, D9766) was first diluted in 400 μL of DMSO (Cayman, 71210) and then in 2 mL of 100% ethanol. Sonication was performed in a water bath until it was completely dissolved. Finally, 17.6 mL of corn oil was added to the solution for a final working concentration of 1 mg/mL (19 (link)). The solution was kept in the dark due to light sensitivity. Mice were pretreated with Dipyridamole, 5 mg/kg, i.p. 60 minutes prior to the onset of myocardial ischemia. The control groups were given the same amount of PBS.

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Ruan W., Li J., Choi S., Ma X., Liang Y., Nair R., Yuan X., Mills T.W, & Eltzschig H.K. (2023). Targeting myocardial equilibrative nucleoside transporter ENT1 provides cardioprotection by enhancing myeloid Adora2b signaling. JCI Insight, 8(11), e166011.

Publication 2023

Dipyridamole DMSO

Bath Cayman Corn oil Dipyridamole Dmso Ethanol Light sensitivity Mice Myocardial ischemia

Corresponding Organization : The University of Texas Health Science Center at Houston

Other organizations : Central South University, Second Xiangya Hospital of Central South University, Zhejiang University, Sir Run Run Shaw Hospital

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Variable analysis

independent variables

Dipyridamole administration, 5 mg/kg, i.p. 60 minutes prior to the onset of myocardial ischemia

dependent variables

Outcome of myocardial ischemia

control variables

The control groups were given the same amount of PBS

controls

Positive control: Mice pretreated with dipyridamole
Negative control: Mice given the same amount of PBS

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