Quantifying Residual PEG in Fiber Mats

¹H-NMR spectra of the fiber mats were obtained using a 500 MHz NMR (Varian, Palo Alto, CA, USA) to quantify the percent of residual PEG after washing. To obtain the NMR spectra, PEG was dissolved in D₂O and others were dissolved in DMSO-d6. DSC scans of the mats were obtained using a Mettler DSC 823e instrument (Mettler Toledo Inc., Columbus OH, USA). The heating rate was 10 °C min⁻¹.^{30 (link)} Heat of fusion was determined from the DSC scans as the area under the melting peak using a base line extending to ~10 °C on either side of the PEG melting peak at 60 °C.

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Goyal R., Vega M.E., Pastino A.K., Singh S., Guvendiren M., Kohn J., Murthy N.S, & Schwarzbauer J.E. (2017). Development of hybrid scaffolds with natural extracellular matrix deposited within synthetic polymeric fibers. Journal of biomedical materials research. Part A, 105(8), 2162-2170.

Publication 2017

500 MHz NMR DSC 823e

1h nmr Base line Dmso Fiber Scans

Corresponding Organization : Princeton University

Other organizations : Rutgers, The State University of New Jersey, New Jersey Institute of Technology

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Variable analysis

independent variables

None explicitly mentioned

dependent variables

Percent of residual PEG after washing
Heat of fusion

control variables

Heating rate (10 °C min^-1)
NMR spectra obtained using a 500 MHz NMR (Varian, Palo Alto, CA, USA)
DSC scans obtained using a Mettler DSC 823e instrument (Mettler Toledo Inc., Columbus OH, USA)

controls

PEG was dissolved in D2O
Others were dissolved in DMSO-d6

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