The method was as described in our previous study [19 (link)]. ROS levels in RAECs were measured by a DCFH-DA fluorescent probe according to the ROS assay kit protocol (Beyotime, China). TUNEL staining of paraffin sections was performed according to the TUNEL kit protocol (Beyotime, China). JC-1 staining of RAECs was performed according to the JC-1 assay kit protocol (Solarbio, China).
Free full text: Click here