The peptides were synthesized by the N–9–fluorenylmethyloxycarbonyl (Fmoc) strategy using a Liberty Blue automated microwave peptide synthesizer (CEM, Stallings, NC, USA), as previously described [12 (link)]. The purification of the peptides was accomplished by liquid chromatography using an AKTA pure chromatography system (GE Healthcare, Chicago, Illinois, USA) with purity >95%. The sequence and degree of purity (>95%) were confirmed by mass spectrometry by an ULTRAFLEX MALDI–TOF/TOF mass spectrometer (Bruker, Fremont, CA, USA).
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