Matrigel Assay for Evaluating Angiogenesis

Tube formation ability was evaluated using a Matrigel assay (BD, USA). Matrigel (9.2 mg/ml) was used at 1:1 dilution with a completed medium. ECs were seeded on Matrigel with VEGF (R&D, CDN) (20 ng/ml) and tofacitinib (1 μM). The concentration of tofacitinib followed what had already been published in similar experimental models [21 (link)–23 ]. After 4 h, the images were acquired using an Olympus BX53 microscope. The total tube length, the total branching length and the number of junctions of each experiment were measured, using NIH ImageJ. Results were expressed as median (range).

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Di Benedetto P., Ruscitti P., Berardicurti O., Panzera N., Grazia N., Di Vito Nolfi M., Di Francesco B., Navarini L., Maurizi A., Rucci N., Teti A.M., Zazzeroni F., Guggino G., Ciccia F., Dolo V., Alesse E., Cipriani P, & Giacomelli R. (2021). Blocking Jak/STAT signalling using tofacitinib inhibits angiogenesis in experimental arthritis. Arthritis Research & Therapy, 23, 213.

Publication 2021

Matrigel assay VEGF BX53 microscope

Assay Dilution Matrigel Microscope Tofacitinib Vegf d

Corresponding Organization :

Other organizations : University of L'Aquila, Università Campus Bio-Medico, University of Palermo, University of Campania "Luigi Vanvitelli"

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Variable analysis

independent variables

Tofacitinib (1 μM)

dependent variables

Total tube length
Total branching length
Number of junctions

control variables

Matrigel (9.2 mg/ml) used at 1:1 dilution with a completed medium
ECs seeded on Matrigel with VEGF (R&D, CDN) (20 ng/ml)
Images acquired using an Olympus BX53 microscope after 4 h

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