Virulent, Recombinant, and Attenuated DPV Strains

The virulent strain of DPV, CHv, was isolated and characterized by our lab (32 (link)). The recombinant virulent strain of DPV, BAC-CHv-EGFP (CHv-GFP), was constructed by our research center (33 (link)). The attenuated vaccine strain of DPV, CHa, was retrieved from storage at our research center. The Duck Tambusu virus (DTMUV) was stored at our research center. Each DPV and DTMUV strain was propagated on DEFs. The Rabbit anti-human MAP2 and -human GFAP polyclonal antibody and the goat anti-human β-actin monoclonal antibody were purchased from Abclonal (Wuhan, China). Mouse anti-duck CD80 (Gene ID:101796029) and CD86 (Gene ID:101794331) antibodies were generated by our research center. Ruxolitinib, poly(dA:dT), and poly(I:C) were purchased from Invivogen (Hong Kong, China).

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Tian B., Cai D., He T., Deng L., Wu L., Wang M., Jia R., Zhu D., Liu M., Yang Q., Wu Y., Zhao X., Chen S., Zhang S., Huang J., Ou X., Mao S., Yu Y., Zhang L., Liu Y, & Cheng A. (2020). Isolation and Selection of Duck Primary Cells as Pathogenic and Innate Immunologic Cell Models for Duck Plague Virus. Frontiers in Immunology, 10, 3131.

Publication 2019

Ruxolitinib poly(dA:dT), poly(I:C)

Actin Anti antibody Anti cd80 Antibodies Antibody Attenuated vaccine Duck Gene Gfap Goat Human Map2 Monoclonal antibody Mouse Poly Poly i c Rabbit Ruxolitinib Strain Virus

Corresponding Organization : Sichuan Agricultural University

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Variable analysis

independent variables

Virulent strain of DPV, CHv
Recombinant virulent strain of DPV, BAC-CHv-EGFP (CHv-GFP)
Attenuated vaccine strain of DPV, CHa
Duck Tambusu virus (DTMUV)
Ruxolitinib
Poly(dA:dT)
Poly(I:C)

dependent variables

Propagation of DPV and DTMUV strains on DEFs

control variables

DEFs used for propagation of DPV and DTMUV strains

controls

Positive control: Not specified
Negative control: Not specified

Annotations

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