High-Resolution Cryo-EM of Actomyosin

Fig. S1 and Fig. S2 describe the workflows to generate the actomyosin helical and focused single particle reconstructions. Fig. S3 contains the local resolution plots for each reconstruction and presents the map quality for representative regions. Fig. S4 shows a comparison of the rigor and ADP-bound actomyosin models. Insets of the actomyosin interface for each model highlight the conservation of the binding interface. Fig. S5 demonstrates the improved quality and resolution of the focused refinements in the outer regions of the myosin motor and essential light chains for both the rigor and ADP-bound forms.