Protocol detail

Lipid Autacoids Extraction and Analysis

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Lipid autacoids were extracted by solid phase with SampliQ ODS-C18 cartridges (Agilent Technologies). Eicosanoids and docosanoids were identified and quantified using a triple-quadrupole linear ion trap LC/MS/MS system (MDS SCIEX 3200 QTRAP) equipped with a Kinetex C18 mini-bore column.

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von Moltke J., Trinidad N.J., Moayeri M., Kintzer A.F., Wang S.B., van Rooijen N., Brown C.R., Krantz B.A., Leppla S.H., Gronert K, & Vance R.E. (2012). Rapid induction of inflammatory lipid mediators by the inflammasome in vivo. Nature, 490(7418), 107-111.

Publication 2012

Autacoids Eicosanoids Lipid Ms ms

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Protocol cited in 11 other protocols

Variable analysis

independent variables

Solid phase extraction method (SampliQ ODS-C18 cartridges)

dependent variables

Identification and quantification of eicosanoids and docosanoids

control variables

Triple-quadrupole linear ion trap LC/MS/MS system (MDS SCIEX 3200 QTRAP)
Kinetex C18 mini-bore column

controls

No positive or negative controls were explicitly mentioned.

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