Proteomic Analysis of Stress Response

BTD and DiaAlk were homemade; Catalase (C9322), Iodoacetamide (V900335), Tris[(1-benzyl-1H-1,2,3-triazol-4-yl)methyl]amine (TBTA) (678937), and sodium ascobate (A7631) were purchased from Sigma-Aldrich; dithiothreitol (A100281) was purchased from BBI Life Sciences; IPM (EVU111), Light Azido-UV-Biotin (EVU102), and Heavy Azido-UV-Biotin (EVU151) were purchased from KeraFast; CuSO₄ (C493-500) was purchased from Thermo Fisher Scientific; Sequencing grade trypsin (V5113) was purchased from Promega. Anti-actin antibody (A5441) (1:500) and anti-tubulin antibody (T9026) (1:500) were purchased from Sigma-Aldrich; Anti-p-eIF2α (3597) (1:1000) and anti-p-p38 (9211) (1:500) antibodies were purchased from Cell Signaling Technology; Anti-PMK-1 antibody (1:500) was a kind gift from Dr. R. Pukkila-Worley at University of Massachusetts Medical School, Worcester, MA^{58 (link)}; Anti-PRDX-2 antibody (1:2000) was a kind gift from Dr. E. Veal at Newcastle University Biosciences Institute, Newcastle upon Tyne, UK^{30 (link)}.