MFH-loaded COM/Na-Alg
microspheres were formulated via the ionotropic
gelation method with the cross-linker being calcium chloride (CaCl2). The aqueous dispersion of COM was prepared with Na-Alg
in various proportions (Table 1 ), in distilled water with constant stirring at 1000 rpm for
30 min.11 (link) Later, MFH was added to this
polymeric mixture keeping the drug concentration constant, i.e., 100
mg in each formulation. The homogenization of the suspension was carried
out for 20 min at 1000 rpm speed and debubbled by ultrasonication.
This mixture was then taken in a dispensable syringe with a No. 22
needle and added dropwise to the solution of cross-linker, i.e., CaCl2 (10%), for curing reaction completion. The rigid microspheres
formed were kept in the CaCl2 solution for 30 min, filtered,
and washed with distilled water twice. The prepared COM-Na-Alg microspheres
were oven-dried at 40 °C for 24 h and stored in a desiccator
for further use.24
microspheres were formulated via the ionotropic
gelation method with the cross-linker being calcium chloride (CaCl2). The aqueous dispersion of COM was prepared with Na-Alg
in various proportions (
30 min.11 (link) Later, MFH was added to this
polymeric mixture keeping the drug concentration constant, i.e., 100
mg in each formulation. The homogenization of the suspension was carried
out for 20 min at 1000 rpm speed and debubbled by ultrasonication.
This mixture was then taken in a dispensable syringe with a No. 22
needle and added dropwise to the solution of cross-linker, i.e., CaCl2 (10%), for curing reaction completion. The rigid microspheres
formed were kept in the CaCl2 solution for 30 min, filtered,
and washed with distilled water twice. The prepared COM-Na-Alg microspheres
were oven-dried at 40 °C for 24 h and stored in a desiccator
for further use.24
