ADAP-Deficient Mouse Models Generation

C57BL/6 (B6) wild-type and ADAP^−/− mice were generated as previously described (17 (link)). P14 ADAP^−/− Rag2^−/− mice were generated by crossing ADAP^−/− mice with P14 Rag2^−/− mice (provided by Dr. S. Jameson, University of Minnesota). OT-I ADAP^−/− mice were generated by crossing ADAP^−/− mice with OT-I mice (The Jackson Laboratory, Bar Harbor, ME). Nur77-GFP ADAP^−/− mice were generated by crossing ADAP^−/− mice with Nur77-GFP mice (provided by Dr. K. Hogquist and Dr. M. Farrar, University of Minnesota) (18 (link)). All mice were harvested between 8–12 weeks of age, unless otherwise specified. B6 CD45.1, B6 CD45.2 and Rag1^−/− CD45.2 recipient mice were purchased from The Jackson Laboratory. IL-15^−/− recipient mice were kindly provided by Dr. M. Farrar and Dr. D. Masopust, University of Minnesota. Mice were housed in specific pathogen-free facilities at the University of Minnesota. All experimental protocols involving the use of mice were approved by the Institutional Animal Care and Use Committee at the University of Minnesota.