Cell Line Cultivation for Breast Cancer Research

MDA-MB-231 cells were obtained from American Tissue Culture Collection. Cancer associated fibroblasts were isolated from invasive breast ductal carcinoma and characterized in previous studies [95 (link), 96 (link)]. DCIS.com cells were generously provided by Fariba Behbod, PharmD, Ph.D (University of Kansas Medical Center). Raw 264.7 mcherry cells were generously provided by George Veilhauer, Ph.D (University of Kansas Medical Center, Kansas City, KS). All cell lines were cultured on plastic in DMEM media containing 10% FBS with 0.1% amphotericin, 1% penicillin-streptomycin (cat no. 30-004-CI, Cellgro).

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Fang W.B., Yao M., Brummer G., Acevedo D., Alhakamy N., Berkland C, & Cheng N. (2016). Targeted gene silencing of CCL2 inhibits triple negative breast cancer progression by blocking cancer stem cell renewal and M2 macrophage recruitment. Oncotarget, 7(31), 49349-49367.

Publication 2016

MDA-MB-231 cells amphotericin penicillin-streptomycin

Amphotericin Cancer associated fibroblasts Cell lines Cells Dcis Invasive breast ductal carcinoma Mda mb 231 cells Penicillin Raw 264 7 cells Streptomycin

Corresponding Organization : University of Kansas Medical Center

Other organizations : University of Kansas

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Variable analysis

independent variables

Cell lines used: MDA-MB-231 cells, cancer associated fibroblasts, DCIS.com cells, Raw 264.7 mcherry cells

dependent variables

No dependent variables explicitly mentioned

control variables

All cell lines were cultured on plastic in DMEM media containing 10% FBS with 0.1% amphotericin, 1% penicillin-streptomycin

controls

No positive or negative controls explicitly mentioned

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