Immunoblotting Protocol for Myelin Proteins

Immunoblotting was performed as described (Patzig et al., 2016a (link); Schardt et al., 2009 (link)). Antibodies were specific for ANLN (Acris AP16165PU-N; 1:1000), SEPT2 (ProteinTech Group 11397–1-AP; 1:500), SEPT4 (IBL JP18987; 1:500), SEPT7 (IBL JP18991; 1:5000), SEPT8 (ProteinTech Group 11769–1-AP; 1:2500), MAG ((Erb et al., 2003 (link)); kindly provided by N. Schaeren-Wiemers, Basel; 1:500), PLP/DM20 (A431 ((Jung et al., 1996 (link)); 1:5000), cyclic nucleotide phosphodiesterase (CNP) (Sigma C5922; 1:1000), MOG (clone 8–18 C5 (Linnington et al., 1984 (link)); 1:5000; kindly provided by C. Linington, Glasgow), SIRT2 (abcam 67299; 1:500), CD9 (abcam ab92726; 1:2000), CA2 ((Ghandour et al., 1980a (link); Ghandour et al., 1980b (link)); 1:1000; kindly provided by S. Ghandour, Strasbourg), ATP1A1 (abcam ab7671; 1:2500), ATP1A3 (abcam ab2826; 1:1000), beta3-Tubulin (TUBB3/Tuj1) (Covance MMS-435P; 1:1000). Secondary HRP-coupled antibodies were anti-mouse (dianova 715-035-020; 1:1000), -rabbit (dianova 711-035-152; 1:1000), or -goat (dianova 705-035-003; 1:500). Immunoblots were scanned using the Intas ChemoCam system.