Palmitate–TMS derivative was analyzed using an Agilent 5973N-MSD equipped with an Agilent 6890 GC system, and a DB-17MS capillary column (30 m x 0.25 mm × 0.25 µm). The mass spectrometer was operated in the electron impact mode (EI; 70 eV). The temperature program was as follows: 100 °C initial, increase by 15 °C/min to 295 °C and hold for 8 min. The sample was injected at a split ratio of 10:1 with a helium flow of 1 mL/min. Palmitate–TMS derivative eluted at 9.7 min. Mass scan from 100 to 600 was chosen in the method. The m/z at 313, 314, and 327 were extracted for M0/M1 palmitate and heptadecanoic acid quantification.
All the stable isotope labeling was corrected from the natural stable isotope distribution37 (link). The newly synthesized total palmitic acid was calculated as following:36 (link) %newly synthesized palmitic acid labeling = total palmitic acid labeling /(plasma 2H2O labeling × 22) × 100.
All the stable isotope labeling was corrected from the natural stable isotope distribution37 (link). The newly synthesized total palmitic acid was calculated as following:36 (link) %newly synthesized palmitic acid labeling = total palmitic acid labeling /(plasma 2H2O labeling × 22) × 100.
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