Plasma Arginine Availability Quantification

Plasma samples were prepared using a Waters AccQTag derivatization kit (Manchester, UK) and subjected to ultra-performance liquid chromatography (UPLC) separation using the ACQUITY UPLC system (Waters). A multiple reaction monitoring (MRM) analysis was performed using a Xevo TQ-XS (Waters) mass spectrometer. Data were analyzed using Waters MassLynx 4.2 software and quantified using TargetLynx. Arginine availability index (AAI) was used to evaluate the relative availability of Arg and was calculated as [Arg]/([ornithine] + [lysine]) [29 (link)].

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Yeh C.L., Tanuseputero S.A., Wu J.M., Tseng Y.R., Yang P.J., Lee P.C., Yeh S.L, & Lin M.T. (2020). Intravenous Arginine Administration Benefits CD4+ T-Cell Homeostasis and Attenuates Liver Inflammation in Mice with Polymicrobial Sepsis. Nutrients, 12(4), 1047.

Publication 2020

AccQTag derivatization kit ACQUITY UPLC system Xevo TQ-XS MassLynx 4.2 software

Arginine Liquid chromatography Lysine Ornithine Plasma

Corresponding Organization : National Taiwan University Hospital

Other organizations : Taipei Medical University, Taipei Medical University Hospital

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Variable analysis

independent variables

Plasma sample preparation using Waters AccQTag derivatization kit

dependent variables

Arginine availability index (AAI)

control variables

UPLC separation using the ACQUITY UPLC system (Waters)
MRM analysis using a Xevo TQ-XS (Waters) mass spectrometer
Data analysis using Waters MassLynx 4.2 software and TargetLynx

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