A WST-8 Cell Counting Kit (Wako, Osaka, Japan) was used to assess the proliferation of cells grown in 96-well microplates. Prostate cancer cells were seeded in DMEM medium containing 10% fetal bovine serum (FBS) in 96-well plates (5 × 103 cells/well), and a WST-8 assay was performed as previously described [16 (link)].
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