SDS-PAGE and Western Blot Analysis

Proteins were separated on SDS–Page in a Mini Protean apparatus (Bio-Rad, Hercules, CA, USA) [54 (link)] and then electroblotted on PVDF membrane with the Trans–Blot Turbo Transfer System (Bio-Rad, Hercules, CA, USA), according to the manufacturer’s instructions. Anti-STRP [7 (link)] 1:10,000, anti-actin (Agrisera, Vännäs, Sweden) 1:5000, and anti-GFP (Santa Cruz Biotechnology, Inc., Heidelberg, Germany, DE) 1:1000 dilutions were used. Protein detections were performed by incubating the membrane with horseradish peroxidase–conjugated anti-rabbit secondary antibody (1:5000) or anti-mouse secondary antibody (1:5000) from Bio-Rad.

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Fiorillo A., Manai M., Visconti S, & Camoni L. (2023). The Salt Tolerance–Related Protein (STRP) Is a Positive Regulator of the Response to Salt Stress in Arabidopsis thaliana. Plants, 12(8), 1704.

Publication 2023

Mini Protean Trans–Blot Turbo Transfer System anti-actin anti-GFP horseradish peroxidase–conjugated anti-rabbit secondary antibody

Actin Anti antibody Dilutions Horseradish peroxidase Membrane Mouse Protein Pvdf Rabbit Sds page

Corresponding Organization : University of Rome Tor Vergata

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Variable analysis

independent variables

SDS–Page separation of proteins in a Mini Protean apparatus (Bio-Rad, Hercules, CA, USA)
Electroblotting of proteins on PVDF membrane with the Trans–Blot Turbo Transfer System (Bio-Rad, Hercules, CA, USA)

dependent variables

Protein detections using anti-STRP, anti-actin, and anti-GFP primary antibodies
Protein detections using horseradish peroxidase–conjugated secondary antibodies

control variables

Manufacturer's instructions for the Mini Protean apparatus and Trans–Blot Turbo Transfer System
Antibody dilutions (anti-STRP 1:10,000, anti-actin 1:5000, anti-GFP 1:1000, secondary antibodies 1:5000)

controls

No positive or negative controls were explicitly mentioned in the provided information.

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