Terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) (TACS2 TdT DAB, Trevigen) staining was used as a measure of apoptosis in tissues as previously described [85 (link)]. Apoptosis was quantified by Flow Cytometry using the Annexin V-Cy5 apoptosis detection kit, as per the manufacturer’s instructions (Abcam).
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