Biochemical Markers in Hemodialysis Patients

Approximately 5 ml of the blood sample was collected before hemodialysis from all participants. The samples were immediately centrifuged at 3000g for 10 min and stored at 4°C within one hour of collection for downstream biochemical analyses. Serum levels of total cholesterol (TCH), triglycerides, albumin, globulin, blood urea nitrogen, creatinine, glucose, total calcium, and phosphorus were measured using an autoanalyzer (Siemens Advia 1800, Siemens Healthcare, Henkestr, Germany). Serum leptin concentrations were measured using a commercially available enzyme immunoassay kit (SPI-BIO, Montigny le Bretonneux, France) [19 (link), 20 (link)]. Serum intact parathyroid hormone (iPTH) levels were measured using an autoanalyzer (Siemens Advia Centaur XP immunoassay system, Siemens Healthcare).

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Wang C.H., Lai Y.H., Lin Y.L., Kuo C.H., Syu R.J., Chen M.C, & Hsu B.G. (2020). Increased Serum Leptin Level Predicts Bone Mineral Density in Hemodialysis Patients. International Journal of Endocrinology, 2020, 8451751.

Publication 2020

Siemens Advia 1800 Siemens Advia Centaur XP immunoassay system

Albumin Blood Blood urea nitrogen Calcium Cholesterol Creatinine Enzyme immunoassay Globulin Glucose Hemodialysis Immunoassay Leptin Parathyroid hormone Phosphorus Serum Triglycerides

Corresponding Organization : Tzu Chi University

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Protocol cited in 2 other protocols

Variable analysis

independent variables

None explicitly mentioned

dependent variables

Serum levels of total cholesterol (TCH)
Serum levels of triglycerides
Serum levels of albumin
Serum levels of globulin
Serum levels of blood urea nitrogen
Serum levels of creatinine
Serum levels of glucose
Serum levels of total calcium
Serum levels of phosphorus
Serum leptin concentrations
Serum intact parathyroid hormone (iPTH) levels

control variables

Amount of blood sample collected (approximately 5 ml)
Centrifugation speed (3000g)
Centrifugation duration (10 minutes)
Storage temperature (4°C)
Time between sample collection and storage (within one hour)

positive controls

None mentioned

negative controls

None mentioned

Annotations

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