Quantifying Pancreatic Endocrine Cell Types

Islets were defined as groups of >20 endocrine cells^{56 (link)–58 (link)} and smaller groups were defined as clusters. Estimation of the relative pancreatic area occupied by SYN, INS, or CHYM cells was performed as described^{53 (link)}, using a Nanozoomer Digital Pathology slide scanner (Hamamatsu; Bridgewater, New Jersey) at the Cellular and Molecular Imaging Core, DRC University of Washington, and the Visiopharm software (Hoersholm, Denmark).

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Bogdani M., Blackman S.M., Ridaura C., Bellocq J.P., Powers A.C, & Aguilar-Bryan L. (2017). Structural abnormalities in islets from very young children with cystic fibrosis may contribute to cystic fibrosis-related diabetes. Scientific Reports, 7, 17231.

Publication 2017

Nanozoomer Digital Pathology slide scanner

Cellular Endocrine Pancreatic Scanner

Corresponding Organization : Pacific Northwest Diabetes Research Institute

Other organizations : Johns Hopkins Medicine, Johns Hopkins University, Instituto Nacional de Pediatria, Hôpital d'Hautepierre, VA Tennessee Valley Healthcare System, Vanderbilt University

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Variable analysis

independent variables

None explicitly mentioned

dependent variables

Relative pancreatic area occupied by SYN, INS, or CHYM cells

control variables

None explicitly mentioned

positive controls

None specified

negative controls

None specified

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