In a soundproof chamber (O’Hara & Co.), ultrasonic sounds were detected by a condenser ultrasound microphone (UltraSoundGate CM16/CMPA, Avisoft Bioacoustics, Berlin, Germany) connected to an A/D converter (UltraSoundGate 116Hb, Avisoft Bioacoustics). Acoustic signals were recorded with an Avisoft SASLab Pro Recorder (Avisoft Bioacoustics) and transmitted to a sound analysis system (Avisoft SASLab Pro, Avisoft Bioacoustics).
To evaluate communication-related behaviour during postnatal stages, we monitored maternal separation-induced USVs as described previously34 (link) with some modifications. Each pup was isolated from their mother and placed in a small cage containing fresh bedding. The number of USVs for 5 min was counted manually.
To examine adult vocal communication during different-sex interactions, we monitored courtship USVs as described previously39 (link). To induce courtship USVs in male mouse, an oestrous female C57BL/6J mouse was introduced into the male home cage. The number of USVs in a 5-min period was counted manually.
To further examine adult vocal communication during same-sex social interactions, we monitored USVs in the resident-intruder test as described previously28 (link)–30 (link) with some modifications. An intruder C57BL/6J mouse was introduced to the resident home cage. The number of USVs in a 5-min period was counted manually.
To evaluate communication-related behaviour during postnatal stages, we monitored maternal separation-induced USVs as described previously34 (link) with some modifications. Each pup was isolated from their mother and placed in a small cage containing fresh bedding. The number of USVs for 5 min was counted manually.
To examine adult vocal communication during different-sex interactions, we monitored courtship USVs as described previously39 (link). To induce courtship USVs in male mouse, an oestrous female C57BL/6J mouse was introduced into the male home cage. The number of USVs in a 5-min period was counted manually.
To further examine adult vocal communication during same-sex social interactions, we monitored USVs in the resident-intruder test as described previously28 (link)–30 (link) with some modifications. An intruder C57BL/6J mouse was introduced to the resident home cage. The number of USVs in a 5-min period was counted manually.
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