Example 11
50 μmol (Gd3+) of the AGuIX® nanoparticles were redispersed in 250 μl of ultrapure water in order to obtain a solution at 200 mM ([Gd3+]). 0.6 mg of tyr3-octreotate (TATE) peptide are placed in a 2.5 ml flask. 20 μl of ultrapure water are added to the flask, which is stirred until the peptide has completely dissolved. A solution containing 30 g/l of peptide is then obtained. 20 μl of this solution are then added to the solution of AGuIX®, as are 230 μl of ultrapure water. The flask is stirred for 30 minutes. A solution containing 100 mM of gadolinium and 1.20 g/l of peptide is thus obtained.
This solution is placed in a 3 kDa Vivaspin®, and a tangential filtration cycle is carried out so as to obtain a supernatant of 320 μl. The subnatant is analysed by UV-visible analysis (20-fold dilution) or fluorometry (40-fold dilution).
