Influenza A nucleoprotein was detected in the oropharyngeal swab collected from one of the virus-inoculated dogs at 24 hours p.i. The oropharyngeal swabs collected from one dog at 72, 84, and 120 hours p.i., and another dog at 108, 120, and 132 hours p.i., were positive for virus by quantitative real-time RT-PCR (Table 11). The absolute number of influenza M gene copies per μL of swab extract increased with time from 3 to 6 days p.i. No virus was detected in the rectal swabs.
US11865172B2. Materials and methods for respiratory disease control in canines (2024-01-09). UNIVERSITY OF FLORIDA RESEARCH FOUNDATION, INC. [US], CORNELL RESEARCH FOUNDATION, INC. [US], The Government of The United States of America as represented by The Secretary of the Department of [US]. Inventors: Patti Cynthia Crawford [US], Paul J. Gibbs [US], Edward J. Dubovi [US], Ruben Omar Donis [US], Jacqueline Katz [US], Alexander I. Klimov [US], Nallakannu P. Lakshmanan [US], Melissa Anne Lum [US], Daniel Ghislena Emiel Goovaerts [NL], Mark William Mellencamp [US], Nancy J. Cox [US], William L. Castleman [US].
Patent 2024
GeneInfluenzaNucleoproteinOropharyngealQuantitative real time pcrRectalVirusVirus influenza a
Time of oropharyngeal swab collection (24, 72, 84, 108, 120, 132 hours post-inoculation (p.i.))
dependent variables
Presence of influenza A nucleoprotein in oropharyngeal swabs (detected by quantitative real-time RT-PCR)
Absolute number of influenza M gene copies per μL of swab extract
control variables
Rectal swabs (no virus detected)
controls
Positive control: Not explicitly mentioned
Negative control: Not explicitly mentioned
Annotations
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