Genetic Mouse Models for Hemophilia A

Wild-type C57BL/6J (WT) mice were purchased from Japan SLC (Shizuoka, Japan). F8^fl/fl mice possessing 2 loxP sites flanking exons 17–18 (F8^tm1Rmnt/J)^{13 (link)}, Alb-Cre mice (B6.Cg-Tg(Alb-Cre)21Mgn/J)^{44 (link)}, and lymphatic vascular endothelial hyaluronan receptor-1 (Lyve1)-Cre (B6;129P2-Lyve1^{tm1.1(EGFP/cre)Cys}/J) mice^{45 (link)} were obtained from The Jackson Laboratory (Sacramento, CA, USA). Tie-2-Cre mice (B6.Cg-Tg(Tek-Cre)1Ywa)^{46 (link)} and CAG-Cre mice (B6.Cg-Tg(CAG-Cre)CZ-MO2Osb)^{47 (link)} were obtained from RIKEN BRC (Ibaraki, Japan). The Institutional Animal Care and Concern Committee of Jichi Medical University approved all animal procedures (Approval Number: 17117-13), and animal care was performed according to the committee’s guidelines and as per ARRIVE Guidelines/Checklist.

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Hayakawa M., Sakata A., Hayakawa H., Matsumoto H., Hiramoto T., Kashiwakura Y., Baatartsogt N., Fukushima N., Sakata Y., Suzuki-Inoue K, & Ohmori T. (2021). Characterization and visualization of murine coagulation factor VIII-producing cells in vivo. Scientific Reports, 11, 14824.

Publication 2021

Alb-Cre mice (B6.Cg-Tg(Alb-Cre)21Mgn/J)

Animal Endothelial Exons Hyaluronan receptor Lymphatic vascular Mice

Corresponding Organization : Jichi Medical University

Other organizations : University of Yamanashi Hospital

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Variable analysis

independent variables

Mouse genotype: Wild-type C57BL/6J (WT), F8^(fl/fl), Alb-Cre, Lyve1-Cre, Tie-2-Cre, CAG-Cre

dependent variables

Not explicitly mentioned

control variables

Not explicitly mentioned

controls

Positive control: Wild-type C57BL/6J (WT) mice
Negative controls: Not explicitly mentioned

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