Protocol detail

Nanoparticle Characterization by SEM, DLS, and FTIR

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A Malvern Zetasizer NanoSeries was used to measure the size distribution and ζ-potential of the samples. In addition, SEM (JEOL JSM-7800F, Pleasanton, CA, USA) was used to evaluate the morphology of the nanoparticles. Samples were diluted 1 to 10 before deposition on an aluminum film. FTIR spectra were collected on a Spectrum spectrometer (Perkin Elmer Inc., Waltham, MA, USA) equipped with a single attenuated total reflectance (ATR) diamond set to a range of 400 cm⁻¹ to 4000 cm⁻¹ [31 (link)].

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Luque-Alcaraz A.G., Velazquez-Antillón M., Hernández-Téllez C.N., Graciano-Verdugo A.Z., García-Flores N., Iriqui-Razcón J.L., Silvas-García M.I., Zazueta-Raynaud A., Moreno-Vásquez M.J, & Hernández-Abril P.A. (2022). Antioxidant Effect of Nanoparticles Composed of Zein and Orange (Citrus sinensis) Extract Obtained by Ultrasound-Assisted Extraction. Materials, 15(14), 4838.

Publication 2022

Zetasizer NanoSeries JSM-7800F Spectrum spectrometer

Aluminum Diamond Ftir

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Variable analysis

independent variables

None explicitly mentioned

dependent variables

Size distribution of nanoparticles
Zeta-potential of nanoparticles
Morphology of nanoparticles

control variables

Dilution ratio (1:10) of samples before deposition on aluminum film

controls

No positive or negative controls were explicitly mentioned in the input text.

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