Cell Lysis and Western Blotting

The preparation of cell lysates and western blotting were performed as described previously (75 (link)). The primary antibodies used were anti-GAS41 (sc-393708, Santa Cruz), anti-H2A.Z (ab4174, Abcam; 2718, CST), and anti-α-tubulin (ab18251, Abcam). Luminescence was analyzed on a FUSION SOLO S (Vilber).

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Kikuchi M., Takase S., Konuma T., Noritsugu K., Sekine S., Ikegami T., Ito A, & Umehara T. (2023). GAS41 promotes H2A.Z deposition through recognition of the N terminus of histone H3 by the YEATS domain. Proceedings of the National Academy of Sciences of the United States of America, 120(43), e2304103120.

Publication 2023

ab4174 ab18251 FUSION SOLO S

Corresponding Organization :

Other organizations : RIKEN Center for Biosystems Dynamics Research, Tokyo University of Pharmacy and Life Sciences, Yokohama City University

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Variable analysis

independent variables

Cell lysates preparation
Western blotting

dependent variables

Luminescence

control variables

Primary antibodies used: anti-GAS41 (sc-393708, Santa Cruz), anti-H2A.Z (ab4174, Abcam; 2718, CST), and anti-α-tubulin (ab18251, Abcam)

controls

Positive control: Not mentioned
Negative control: Not mentioned

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