Comprehensive Immunoblotting Protocol

The primary antibodies used were as follows: SMC1A (Bethyl Laboratories, A300-055A), SMC3 (Bethyl Laboratories, A300-060A), acetylated SMC3 (kindly provided by Dr Prasad Jallepalli), RAD21 (SCC1) (Bethyl Laboratories, A300-080A), SCC-112 (PDS5) (Bethyl Laboratories, A300-089A), OBFC1 (STN1) (Novus Biologicals, NBP2-01006), CTC1 (45 (link)), α-tubulin (MilliporeSigma, T-9026), TEN1 (47 (link)), MAD2 (Bethyl Laboratories, A300-301A), α-actinin (Santa Cruz Biotechnology, SC17829), H3 (Cell Signaling Technology, 9715), Flag (Thermo Fisher Scientific, MA1-91878, PA1-984B), PolA1 (Bethyl Laboratories, A302-805A), MCM7 (Santa Cruz Biotechnology, sc22782), p53 (Cell Signaling Technology, 2524S), and p21 (Santa Cruz Biotechnology, sc-6246).

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Schuck P.L., Ball L.E, & Stewart J.A. (2021). The DNA-binding protein CST associates with the cohesin complex and promotes chromosome cohesion. The Journal of Biological Chemistry, 297(3), 101026.

Publication 2021

α-tubulin α-actinin Flag

Actinin Antibodies Biologicals Ctc1 Novus Smc3 α tubulin

Corresponding Organization : University of South Carolina

Other organizations : Medical University of South Carolina

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Variable analysis

independent variables

None explicitly mentioned

dependent variables

Levels/localization of the following proteins: SMC1A, SMC3, acetylated SMC3, RAD21 (SCC1), SCC-112 (PDS5), OBFC1 (STN1), CTC1, α-tubulin, TEN1, MAD2, α-actinin, H3, Flag, PolA1, MCM7, p53, p21

control variables

None explicitly mentioned

controls

Positive control: None mentioned
Negative control: None mentioned

Annotations

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