The carmine alumn stained whole mounts were prepared as described (52 (link)). The fourth inguinal mammary gland were fixed in 10% neutral buffered formalin for paraffin embedding and processed for immunohistochemistry as described (9 (link)). Oil Red O staining of cryosections of mammary glands was performed as described (53 (link)). Stained sections were imaged with a Observer Z1 epifluorescence microscope (Zeiss, Oberkochen, Germany) or with a A1 confocal microsope (Nikon Instruments Inc, Melville, NY).
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Mammary Gland Histological Analysis
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Other organizations : Commonwealth Medical College, University of Scranton, University of Delaware
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Variable analysis
independent variables
- Carmine alumn staining
- 10% neutral buffered formalin fixation for paraffin embedding
- Oil Red O staining of cryosections
- Immunohistochemistry
- Imaging with epifluorescence microscope
- Imaging with confocal microscope
- Not explicitly mentioned
- No positive or negative controls mentioned
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