Immunohistochemistry and Apoptosis Analysis

Immunohistochemistry was performed as described previously 46 (link),47 (link). The following antibodies were used: F4/80 (LS-C96373-100, Lifespan, 1:500 dilution), CD11b (ab6672, Abcam, 1: 1000 dilution) and α-SMA (ab7817, Abcam, 1: 500 dilution). Immunofluorescence TUNEL assay was used to assess apoptosis. Tissue sections were placed and fixed in 4% paraformaldehyde, and incubated with immunofluorescent TUNEL reaction mixture for 1 h in box. DAPI was used to stain nuclei. Immunofluorescence images were obtained in Olympus IX71 microscope (Tokyo, Japan, Leica).

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Li D.J., Sun S.J., Fu J.T., Ouyang S.X., Zhao Q.J., Su L., Ji Q.X., Sun D.Y., Zhu J.H., Zhang G.Y., Ma J.W., Lan X.T., Zhao Y., Tong J., Li G.Q., Shen F.M, & Wang P. (2021). NAD+-boosting therapy alleviates nonalcoholic fatty liver disease via stimulating a novel exerkine Fndc5/irisin. Theranostics, 11(9), 4381-4402.

Publication 2021

LS-C96373-100 ab6672 ab7817

Antibodies Apoptosis Cd11b Dapi Dilution Immunofluorescence Immunofluorescence assay Immunohistochemistry Microscope Nuclei Paraformaldehyde Stain Tissue Tunel

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Variable analysis

independent variables

Antibodies used for immunohistochemistry: F4/80, CD11b, and α-SMA

dependent variables

Apoptosis assessed using immunofluorescence TUNEL assay

control variables

Tissue sections were placed and fixed in 4% paraformaldehyde
Tissue sections were incubated with immunofluorescent TUNEL reaction mixture for 1 h in box
DAPI was used to stain nuclei

controls

Positive control: Not explicitly mentioned
Negative control: Not explicitly mentioned

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