Particle-Induced Calvarial Osteolysis Model

The particles preparation and a wear particle-induced mouse calvarial osteolysis model was generated as previously described[39 (link)]. Briefly, 8-week-old C57BL/6 J mice were randomly divided into different groups. The mice were anesthetized, and the cranial periosteum was separated from the calvarium by sharp dissection. Then, 100 ul of particle suspension contained with or without Ac-YVAD-CMK (Sigma, 0.2 mg/ml), DFO (Sigma, 9 mg/ml) was uniformly spread over the periosteum at the middle suture of the calvarial. The mice were sacrificed 14 days after the operation, and the calvarial were excised for protein extraction, or fixed in 4% paraformaldehyde for micro-CT and histological analysis. No adverse events were found during the generation of mouse calvarial osteolysis model.