Stimulation: Biotin-anti-CD3 (553060), Biotin-anti-CD4 (553728), and Biotin-anti-CD28 (553296) were from BD Biosciences. Immunoprecipitation: anti-Flag (F1804) Sigma Aldrich; anti-SHP-1 (SC287), anti-SHP-2 (SC280) Santa Cruz. Western blotting: anti-pTyr (05321), anti-GST (06332), anti-ERK (06182), anti-SHP-1 (06117) EMD Millipore; anti-pTyr564-SHP-1 (8849), anti-pTyr416-Src (2101) Cell signaling Technology; anti-pTyr319-ZAP-70 (612574), anti-GRB2 (610111) BD Biosciences; anti-LCK (SC433), anti-ZAP-70 (SC574), anti-pERK (SC7383), anti-HA tag (SC805) Santa Cruz; anti-PTPN7 (ab118978) Abcam; anti-Myc tag (M0473) MBL International; anti-SHP-1 (MS1190) Thermo Fisher; anti-pTyr536-SHP-1(SP1571) ECM Biosciences; anti-actin (A5441) Sigma Aldrich; anti-pPLC-γ1 (44-696G) Biosource;, anti-Oxidized PTP active site mAb (MAB2844) R&D Systems. Rabbit polyclonal antisera to THEMIS7 (link) and THEMIS213 (link) have been described. Streptavidin-HRP conjugate was purchased from Sigma Aldrich. Streptavidin was purchased from Southern Biotechnology.
Protocol detail
Immune Receptor Signaling Pathway Analysis
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Corresponding Organization :
Other organizations : Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, Centre de Physiopathologie de Toulouse-Purpan, United States National Library of Medicine
Top 5 similar protocols
Variable analysis
independent variables
- Biotin-anti-CD3 (553060)
- Biotin-anti-CD4 (553728)
- Biotin-anti-CD28 (553296)
- Anti-pTyr (05321)
- Anti-GST (06332)
- Anti-ERK (06182)
- Anti-SHP-1 (06117)
- Anti-pTyr564-SHP-1 (8849)
- Anti-pTyr416-Src (2101)
- Anti-pTyr319-ZAP-70 (612574)
- Anti-GRB2 (610111)
- Anti-pERK (SC7383)
- Anti-PTPN7 (ab118978)
- Anti-pTyr536-SHP-1(SP1571)
- Anti-actin (A5441)
- Anti-pPLC-γ1 (44-696G)
- Anti-Oxidized PTP active site mAb (MAB2844)
- Anti-Flag (F1804)
- Anti-SHP-1 (SC287)
- Anti-SHP-2 (SC280)
- Anti-LCK (SC433)
- Anti-ZAP-70 (SC574)
- Anti-HA tag (SC805)
- Anti-Myc tag (M0473)
- Anti-SHP-1 (MS1190)
- Streptavidin-HRP conjugate
- Streptavidin
- Rabbit polyclonal antisera to THEMIS7
- Rabbit polyclonal antisera to THEMIS213
- Not specified
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