Radiosensitive and Radioresistant NPC Cell Maintenance

Parental (relatively sensitive to IR, has been previously reported [33 (link)]) and radioresistant subpopulations of NPC cells were maintained in Dulbecco’s modified Eagle’s medium (DMEM) (Invitrogen, Waltham, MA, USA) supplemented with 10% fetal bovine serum (FBS; Invitrogen) at 37 °C and 5% CO2. Cells were plated in 6-well or 12-well plates (Corning Inc., Corning, NY, USA) and the plating efficiencies of cell lines used are higher than 90%. Cells are treated with cycloheximide (CHX; 66-81-9, Abcam, Cambridge, MA, USA), MG132 (S2619, Selleck, Houston, TX, USA), N-acetylcysteine (S0077, Beyotime, Jiangsu, China), or MK2206 (S1078, Selleck) according to studies.