All animal experiments were performed under a project license (No. 2021-X17-69), which was reviewed and approved by the Institutional Animal Care and Use Committee of Chinese PLA General Hospital. All experiments were performed in compliance with the national guidelines for the care and use of animal.
Homozygote Opg−/− (Tnfrsf11btm1Smoc, NM-KO-00004) mice and WT mice were purchased from the Shanghai Research Center for Model Organisms. All mice were housed in specific-pathogen-free conditions under a 12/12-h light/dark cycle, and movement and feeding were not restricted. Mouse genotypes were determined via PCR amplification of DNA isolated from their tails. Twelve-week-old male WT and Opg−/− mice were used in our experiments to exclude the influence of hormones in female mice. A random allocation was performed for each genotype.
Sciatic nerve transection procedures were as follows: Mice were anesthetized using an intraperitoneal injection of 1% sodium pentobarbital. The sciatic nerve of the right hind limb was exposed and transected to a length of 10 mm (den group). The sciatic nerve of the left hind limb was exposed without transection (sham group). Body weight and gait of all mice were evaluated at the indicated time points. The mice were sacrificed before denervation, as well as days 3, 7, and 14 post denervation, and the bilateral gastrocnemius (GAS) muscles of all mice were harvested, weighed, and stored according to standard methods. The wet weight ratio was calculated by dividing the weight of the operative side by that of the sham-operated side.
Homozygote Opg−/− (Tnfrsf11btm1Smoc, NM-KO-00004) mice and WT mice were purchased from the Shanghai Research Center for Model Organisms. All mice were housed in specific-pathogen-free conditions under a 12/12-h light/dark cycle, and movement and feeding were not restricted. Mouse genotypes were determined via PCR amplification of DNA isolated from their tails. Twelve-week-old male WT and Opg−/− mice were used in our experiments to exclude the influence of hormones in female mice. A random allocation was performed for each genotype.
Sciatic nerve transection procedures were as follows: Mice were anesthetized using an intraperitoneal injection of 1% sodium pentobarbital. The sciatic nerve of the right hind limb was exposed and transected to a length of 10 mm (den group). The sciatic nerve of the left hind limb was exposed without transection (sham group). Body weight and gait of all mice were evaluated at the indicated time points. The mice were sacrificed before denervation, as well as days 3, 7, and 14 post denervation, and the bilateral gastrocnemius (GAS) muscles of all mice were harvested, weighed, and stored according to standard methods. The wet weight ratio was calculated by dividing the weight of the operative side by that of the sham-operated side.
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