FFPE DNA Extraction and Bisulfite Treatment

Genomic DNA from tissues was extracted using the E.Z.N.A^® FFPE DNA kit (Omega Bio-tek, Inc., Norcross, GA, USA). DNA concentration measurements and bisulfite treatment were performed as previously described (28 (link)).

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Ma H., Chen X., Hu H., Li B., Ying X., Zhou C., Zhong J., Zhao G, & Duan S. (2018). Hypermethylation of MDFI promoter with NSCLC is specific for females, non-smokers and people younger than 65. Oncology Letters, 15(6), 9017-9024.

Publication 2018

E.Z.N.A® FFPE DNA kit

Bisulfite Genomic Tissues Z dna

Corresponding Organization :

Other organizations : Ningbo University, Ningbo No. 2 Hospital

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Variable analysis

independent variables

Extraction of genomic DNA from tissues using the E.Z.N.A® FFPE DNA kit

dependent variables

DNA concentration measurements
Bisulfite treatment

control variables

Extraction method (E.Z.N.A® FFPE DNA kit)
DNA concentration measurement procedure
Bisulfite treatment procedure

controls

Positive control: Not specified
Negative control: Not specified

Annotations

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